we have a problem in LIFT mode in autoflex III MALDI TOF/TOF system. There is no problem in normal MS mode. but when we change the method into the LIFT, it produces a problem in high voltage. The error is written in dialog box like : "high voltage is ramping", and the color of the button is yellow instead of green. The system is getting ready when the high voltage is achieved but it switches from green to yellow in a second suddenly. Hence, we are not able to do MS/MS in our system now.
do you have any suggestion for this problem?