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Problem of Peptide de novo sequencing via ABI 4800 MALDI TOF/TOF

Problem of Peptide de novo sequencing via ABI 4800 MALDI TOF/TOF - Mass Spectrometry Forum

Problem of Peptide de novo sequencing via ABI 4800 MALDI TOF/TOF - Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


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Old 05-07-2010, 04:49 AM
Pipette Filler
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Default Problem of Peptide de novo sequencing via ABI 4800 MALDI TOF/TOF



Hi,

I am new to mass spectrometry. I work on a non-model animal (genome not sequenced) and de novo sequencing appears to be promising in helping me better identify my separated proteins.

However, I can only access to use the ABI 4800 MALDI TOF/TOF, is it possible for this ms to perform de novo sequencing?

The raw data MS files i receive is CAL or T2D format. Is it possible that this machine could generate wiff files (sorry for this stupid question coz i have no idea)? or is there any methods or softwares to convert into wiff files?

Please kidnly give me an idea. Thanks in advance.
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