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Glycan mass spec

Glycan mass spec - Mass Spectrometry Forum

Glycan mass spec - Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


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Old 10-28-2009, 12:48 PM
Pipette Filler
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Default Glycan mass spec



Hi, just a quickie- do glycan groups on glycosylated peptides carry a charge when ionised by ESI-MS (or is it just the peptides)??
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Old 05-17-2010, 02:28 AM
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Default Re: Glycan mass spec

Hi EJ78,

It depends on which kind of glycan you are looking for. While many sugars do not carry any charge, some of them do (take Sialic acid for instance). Says, if the glycan part is simply the core high mannose structure, then the glycan won't contribute any charge to the glycopeptide; otherwise, if the glycan has a terminal sialic acid, then the answer will be "yes, it contributes one negative charge".

Good luck.
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