We have carried out exhaustive 2D gel electrophoresis on rice plant tissues and have identified proteins through in silico data base searches from the average peptide masses of teh mass spectrometry.
However for some protein spots the existing databases brings up no hit and these are apparently the most responsive proteins and look very novel.
Now i have the average pepetide masses of these proteins and looking into Expasy (both Profound and Mascot) gives me just a couple of peptide hits with very low significance probability.
Is there any way that the amino acid sequence from the average pepetide masses be deduced something like using tripsin restriction sites and the molecular masses entangling the possible amino acid sequence or is it just impossible.
Thanks a lot
International Rice Research institute