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TritonX removal

TritonX removal - Mass Spectrometry Forum

TritonX removal - Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


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Old 01-27-2009, 04:23 PM
vix vix is offline
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Default TritonX removal



Hi All,

Any suggestions for an effective way to remove tritonX-100 carryover from my protein extractions prior to MS/MS?

I'm using an SCX fractionation column prior to MS analysis but this is not efficient enough to remove all the detergent. As a result I'm loosing peptide signals from the MALDI MS specra and have a high level of interference which I think is caused by the tritonX.

Please help,

V
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Old 03-25-2009, 11:49 AM
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Default Re: TritonX removal

Normarun a gel, to get rid of the detergents. And then perform the digestion in gel (cut the gel in 10 pieces)
Otherwise if you wnat to do in in solution digestion, check for a compatible buffer without detergents...

regards
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Old 03-30-2009, 09:03 PM
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Default Re: TritonX removal

Hello,

An 'old-fashioned' method for getting rid of Triton X-100, which is quite effective in my experience, is to use Bio-Beads. I don't know whether this will be suitable for MS. You may already know about this!

See, for example,


A rapid method for removal of detergents from protein solution

Tsuneyoshi Horigome and Hiroshi Sugano

Analytical Biochemistry Volume 130, 1983, Pages 393-396

tgd.
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