MALDI-TOF sample preparation

[molecule2005]

I have some samples to submit for MALDI-TOF.

Does anyone know how to submit them?

What buffers should I use?

thanks

[MicroKiller]

Organic solvents i suppose.. you can try 0.1% TFA in 50% acetonitrile..

[ruby]

yes, 0.1% TFA in ACN and use can add 1% formica cid too to finally suspend the petides.

ruby

[acquity]

It depends on your samples.
Generally, samples are mixed with the matrix solution in a 10:1 or 20:1 volume ratio and matrix is generally prepared in 50%ACN, 50% Water and %0.1 TFA. At times on probe washing maybe helpful after drying the samples if you are especially working with salted samples. Please note that MALDI-MS cannot tolerate buffers upper than 100mM concentration and please also keep in mind that if you are working with Na or K containing buffers you will probably see your peaks at their [M+Na] and [M+K] m/z values.

The following paper maybe helpful for you.

"Matrix-assisted laser desorption/ionization mass spectrometry sample preparation techniques designed for various peptide and protein analytes"

Journal of mass spectrometry 1997, vol. 32, no:6, pp. 593-601

Hope this helps

[moleculardude]

Great information.

thanks Acquity