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MALDI-TOF sample preparation

MALDI-TOF sample preparation - Mass Spectrometry Forum

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  #1  
Old 10-19-2006, 10:48 PM
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Question MALDI-TOF sample preparation



I have some samples to submit for MALDI-TOF.

Does anyone know how to submit them?

What buffers should I use?

thanks
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Old 01-29-2007, 10:44 PM
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Default Re: MALDI-TOF sample preparation

Organic solvents i suppose.. you can try 0.1% TFA in 50% acetonitrile..
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Old 01-31-2007, 03:40 AM
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Default Re: MALDI-TOF sample preparation

yes, 0.1% TFA in ACN and use can add 1% formica cid too to finally suspend the petides.

ruby
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Old 03-26-2007, 08:03 PM
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Default Re: MALDI-TOF sample preparation

It depends on your samples.
Generally, samples are mixed with the matrix solution in a 10:1 or 20:1 volume ratio and matrix is generally prepared in 50%ACN, 50% Water and %0.1 TFA. At times on probe washing maybe helpful after drying the samples if you are especially working with salted samples. Please note that MALDI-MS cannot tolerate buffers upper than 100mM concentration and please also keep in mind that if you are working with Na or K containing buffers you will probably see your peaks at their [M+Na] and [M+K] m/z values.

The following paper maybe helpful for you.

"Matrix-assisted laser desorption/ionization mass spectrometry sample preparation techniques designed for various peptide and protein analytes"

Journal of mass spectrometry 1997, vol. 32, no:6, pp. 593-601

Hope this helps
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Old 03-26-2007, 08:50 PM
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Default Re: MALDI-TOF sample preparation

Great information.

thanks Acquity
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