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CHCA or SA in MALDI???

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Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.



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  #1 (permalink)  
Old 11-13-2007, 07:14 PM
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Default CHCA or SA in MALDI???

The rules in MALDI-TOF say:
CHCA matrix for small MW proteins, digested peptides
SA matrix for whole/large MW proteins.

Unfortunately I have really high quality CHCA (Agilent) and low-quality SA (Sigma). Anyone get good results using CHCA on whole undigested proteins?
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  #2 (permalink)  
Old 01-06-2008, 12:58 PM
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Smile Re: CHCA or SA in MALDI???

Quote:
Originally Posted by danfive View Post
The rules in MALDI-TOF say:
CHCA matrix for small MW proteins, digested peptides
SA matrix for whole/large MW proteins.

Unfortunately I have really high quality CHCA (Agilent) and low-quality SA (Sigma). Anyone get good results using CHCA on whole undigested proteins?
Sorry I don't undersand what you have said
Could you provide more information?
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Old 01-06-2008, 01:00 PM
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Default Re: CHCA or SA in MALDI???

Why not use the linear mode?
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