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Mass Spectrometry Forum Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


How to learn Mass spec

How to learn Mass spec - Mass Spectrometry Forum

How to learn Mass spec - Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


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  #1  
Old 09-07-2007, 10:40 PM
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Default How to learn Mass spec



Hi,

I have studied the theory of mass spec instruments.I would like to have a hands on experience on Mass spec and GC instruments. Could anyone suggest me the possible ways to learn? Thank you for responding
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Old 09-10-2007, 07:00 PM
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Default Re: How to learn Mass spec

Mass spec is very easy. The learning part is the troubleshooting, data analysis, and statistical analysis. Sample Prep and Experimental Design are the most important things. The most important thing to know is how to set up an experiment for the Mass Spec, so results are relevant. Mass spec results are not usually validated by a distant independent lab, so you usually want to verify any interesting finds with cross validations studies like Immunohistochemistry, gene-expression...
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Old 09-12-2007, 07:16 PM
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Default Re: How to learn Mass spec

As for hands-on experience, where do you currently work/go to school?
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Old 12-06-2007, 02:52 PM
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Default Re: How to learn Mass spec

Hi, I think attending some MS research group will help. Working with MS instruments is the only way to get both, theory and hand-on practice/experience. Best of the luck.
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Old 12-11-2007, 10:41 PM
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Default Re: How to learn Mass spec

Thanks
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Old 12-13-2007, 07:02 AM
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Default Re: How to learn Mass spec

the MS was not easy, I means the ESI-MS or Moldi-tof for peptide.
For easy sample, the data was very easy to judge.

But when you met some hydrophobic or strong acidic sample, the data become very strange. Our MS guys always judge wrong or showed small right peak.

Many of our correct sample wrong judged for this reason.
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Old 12-13-2007, 04:00 PM
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Default Re: How to learn Mass spec

Quote:
Originally Posted by glbiochem View Post
the MS was not easy, I means the ESI-MS or Moldi-tof for peptide.
For easy sample, the data was very easy to judge.

But when you met some hydrophobic or strong acidic sample, the data become very strange. Our MS guys always judge wrong or showed small right peak.

Many of our correct sample wrong judged for this reason.
Interesting...Do you recall the make and model of your mass spec??
My only tip for MALDI is to use the best matrix CHCA available. For me that comes from Agilent Tech.
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Old 12-14-2007, 06:19 AM
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Default Re: How to learn Mass spec

Shimadzu, LC-MS, from Japan.
We made around 20,000 peptide one year. Our MS guys need to make over 100 peptide (crude or pure)sample analysis per day. Because the peptide sequence diverse quite different, so their character were not the same. For some strong hydrophobic samples, they were really headache.
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Old 12-17-2007, 10:55 PM
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Default Re: How to learn Mass spec

Hi All,

Thank you for your suggestion. I think it is a lot easy when one starts working with it, but until then, it is confusing. Could any one suggest a bible book to follow and master the LC-MS instrument?, I have worked for couple months on LCQ, and found it interesting, so want to learn more.what are the ways to get a hands on experience?, I am not a student anymore.
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Old 12-18-2007, 03:28 PM
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Default Re: How to learn Mass spec

Quote:
Originally Posted by dolphin View Post
Hi All,

Thank you for your suggestion. I think it is a lot easy when one starts working with it, but until then, it is confusing. Could any one suggest a bible book to follow and master the LC-MS instrument?, I have worked for couple months on LCQ, and found it interesting, so want to learn more.what are the ways to get a hands on experience?, I am not a student anymore.
Don't know of any books, here are the search results for LC-MS in cold spring harbor protocols ---> [Only registered users see links. ]
You can most likely get hands on experience at a Proteomics research lab.
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