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Concentration dilution series

Concentration dilution series - Mass Spectrometry Forum

Concentration dilution series - Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


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Old 08-04-2007, 06:14 AM
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Default Concentration dilution series



Hi,
Could anyne suggest, how much water shud be added to 8nano mole/10micro liter of angiotensin to make 40nano mole/ml ? I added 990micro liters of 50:50 ACN:water.But I am confused about concentration.Please confirm the dilution.
Thanks
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Old 12-19-2007, 01:31 PM
ylu ylu is offline
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Default Re: Concentration dilution series

If you took 10 micro L 8 nM/10 micro L and added into 990 micro L 50% ACN, then the final conc. you made was 8 nM/mL instead of 40 nM/mL you wanted. So you may have to take 50 micro L 10 micro L 8 nM/10 micro L and added into 950 micro L 50% ACN to get final 40 nM/mL. See if you agree?
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Old 01-08-2008, 05:15 AM
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Default Re: Concentration dilution series

I guess you are right. Thank you
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