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08-04-2007, 06:14 AM
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 Concentration dilution series Hi, Could anyne suggest, how much water shud be added to 8nano mole/10micro liter of angiotensin to make 40nano mole/ml ? I added 990micro liters of 50:50 ACN:water.But I am confused about concentration.Please confirm the dilution. Thanks        |
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12-19-2007, 12:31 PM
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| Re: Concentration dilution series If you took 10 micro L 8 nM/10 micro L and added into 990 micro L 50% ACN, then the final conc. you made was 8 nM/mL instead of 40 nM/mL you wanted. So you may have to take 50 micro L 10 micro L 8 nM/10 micro L and added into 950 micro L 50% ACN to get final 40 nM/mL. See if you agree? |
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