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dolphin 08-04-2007 06:14 AM

Concentration dilution series
 
Hi,
Could anyne suggest, how much water shud be added to 8nano mole/10micro liter of angiotensin to make 40nano mole/ml ? I added 990micro liters of 50:50 ACN:water.But I am confused about concentration.Please confirm the dilution.
Thanks

ylu 12-19-2007 01:31 PM

Re: Concentration dilution series
 
If you took 10 micro L 8 nM/10 micro L and added into 990 micro L 50% ACN, then the final conc. you made was 8 nM/mL instead of 40 nM/mL you wanted. So you may have to take 50 micro L 10 micro L 8 nM/10 micro L and added into 950 micro L 50% ACN to get final 40 nM/mL. See if you agree?

dolphin 01-08-2008 05:15 AM

Re: Concentration dilution series
 
I guess you are right. Thank you


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