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Lc Ms/ms

Lc Ms/ms - Mass Spectrometry Forum

Lc Ms/ms - Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


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  #1  
Old 08-02-2007, 06:23 AM
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Default Lc Ms/ms



Can I use HPLC and MS separately? Why LC and MS have to be connected though?
Thank you very much everyone
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  #2  
Old 11-29-2007, 01:20 PM
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Default Re: Lc Ms/ms

Quote:
Originally Posted by janemon View Post
Can I use HPLC and MS separately? Why LC and MS have to be connected though?
Thank you very much everyone
You can use it seperately of course. But the reason it's coupled is only the automation. Now you can separate online with the MS.
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Old 12-06-2007, 01:25 PM
ylu ylu is offline
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Default Re: Lc Ms/ms

I think you can. But have to do some configuration on the computer. What HPLC system and MS system/model do you use?
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Old 09-28-2008, 02:41 PM
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Default Re: Lc Ms/ms

sure you can? but do you have other detector to perform detection job?
or else how are you going to so determination/ sepration?
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Old 10-01-2008, 09:15 AM
ylu ylu is offline
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Default Re: Lc Ms/ms

I'm very certain that the LC system and MS detector can be separated either when you purchase them or use them. For example, I'm using Thermo Finnigan TSQ quantum API MS/MS, the LC system was from the same company, Surveyor, recently we sent it for the repair of the autosampler and we have been using the other LC pump and injector for LC separation.
Also some time if I only want to examine the protein profile of my samples, I bypass the sample from ESI-MS/MS detection, for I don't want the complicated or say, ''dirty'' raw sample to go into the ESI ion source, I use the UV detector coupled to the LC system. Of course this sample can still go to ESI-MS/MS detection after the UV detector if you want.
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Old 01-25-2009, 09:44 PM
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Default Re: Lc Ms/ms

What are you trying to do? Do you need to do any chromatography at all? You have to introduce the sample somehow, whether that is GC, LC, MALDI, or the newer DART (perhaps others as well). MALDI and DART are not dependent on a chromatograph of any kind.
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