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-   -   Lc Ms/ms (http://www.molecularstation.com/forum/mass-spectrometry-forum/1847-lc-ms-ms.html)

janemon 08-02-2007 06:23 AM

Lc Ms/ms
 
Can I use HPLC and MS separately? Why LC and MS have to be connected though?
Thank you very much everyone :wub:

StevieWonder 11-29-2007 01:20 PM

Re: Lc Ms/ms
 
Quote:

Originally Posted by janemon (Post 4536)
Can I use HPLC and MS separately? Why LC and MS have to be connected though?
Thank you very much everyone :wub:

You can use it seperately of course. But the reason it's coupled is only the automation. Now you can separate online with the MS.

ylu 12-06-2007 01:25 PM

Re: Lc Ms/ms
 
I think you can. But have to do some configuration on the computer. What HPLC system and MS system/model do you use?

marcus^^ 09-28-2008 02:41 PM

Re: Lc Ms/ms
 
sure you can? but do you have other detector to perform detection job?
or else how are you going to so determination/ sepration?

ylu 10-01-2008 09:15 AM

Re: Lc Ms/ms
 
I'm very certain that the LC system and MS detector can be separated either when you purchase them or use them. For example, I'm using Thermo Finnigan TSQ quantum API MS/MS, the LC system was from the same company, Surveyor, recently we sent it for the repair of the autosampler and we have been using the other LC pump and injector for LC separation.
Also some time if I only want to examine the protein profile of my samples, I bypass the sample from ESI-MS/MS detection, for I don't want the complicated or say, ''dirty'' raw sample to go into the ESI ion source, I use the UV detector coupled to the LC system. Of course this sample can still go to ESI-MS/MS detection after the UV detector if you want.

MoverZ 01-25-2009 09:44 PM

Re: Lc Ms/ms
 
What are you trying to do? Do you need to do any chromatography at all? You have to introduce the sample somehow, whether that is GC, LC, MALDI, or the newer DART (perhaps others as well). MALDI and DART are not dependent on a chromatograph of any kind.


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