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LTQ mass spectrometry - Zoom scan and SIM mode to analyze sample help

LTQ mass spectrometry - Zoom scan and SIM mode to analyze sample help - Mass Spectrometry Forum

LTQ mass spectrometry - Zoom scan and SIM mode to analyze sample help - Discuss and post questions about LC-MS, Q-STAR, MALDI-TOF, and other Mass Spectrometry Questions in the Mass Spec Forum.


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Old 06-15-2007, 01:17 AM
Pipette Filler
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Default seek for help



a new member here, want to find some help from experts in this forum.
It is about the resolution issue of LTQ mass spectrometry, I use Zoom scan, SIM mode to analyze my sample, usually we can observe isotopic peaks for a protein, but these days, these peaks disappear, the resolution seemed to have a severe drop. what is the most likely problem I have met, sample problem or equipment problem?
Thanks in advance!
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Old 06-18-2007, 04:45 PM
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Default Re: LTQ mass spectrometry - Zoom scan and SIM mode to analyze sample help

You could check your helium tank – sounds like it may be out. Other causes of your problem could include electronic failure (although I would expect an “all or nothing” response in this scenario) or perhaps loss of calibration. Hope this helps!
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Old 06-19-2007, 03:24 AM
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Default Re: LTQ mass spectrometry - Zoom scan and SIM mode to analyze sample help

Thanks a lot, it do helps.
Regarding the electronic failure, I observed that all the components and parameters are fine, within the torlance of setting value, so I think it might be ok.
I will check the helium tank first.

Quote:
Originally Posted by TFSTrapMarketeer View Post
You could check your helium tank – sounds like it may be out. Other causes of your problem could include electronic failure (although I would expect an “all or nothing” response in this scenario) or perhaps loss of calibration. Hope this helps!
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analyze , ltq , mass , mode , sample , scan , sim , spectrometry , zoom


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