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Cell Lysis Buffers Compatible with Mass Spectrometry

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Old 06-11-2007, 12:53 PM
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Question Cell Lysis Buffers Compatible with Mass Spectrometry

Hello,

does anyone know of the cell lysis buffers that compatible with Mass Spectrometry?

thanks in advance!
Moleculardude
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Old 12-06-2007, 01:09 PM
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Default Re: Cell Lysis Buffers Compatible with Mass Spectrometry

Hi, I think the crucial step is the digestion and LC separation before introduce to the ion source of MS. see if this paper contains the useful idea for you?
Optimization of MS-Compatible surfactants for Shotgun Proteomic.
Emily I. Chen, et. al.
Journal of Proteome Research. 2007, 6, 2529-2538.
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