Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Lab Equipment Discussion and Reviews (http://www.molecularstation.com/forum/lab-equipment-discussion-reviews/)
-   -   Microtiter plates, antibiotics, bacterial MICs (http://www.molecularstation.com/forum/lab-equipment-discussion-reviews/87019-microtiter-plates-antibiotics-bacterial-mics.html)

Shane.vontelin.van.breda 12-22-2012 06:31 AM

Microtiter plates, antibiotics, bacterial MICs
 
Hey guys, i ahve already posted this in Micorbiology but i think it might also apply here. I am busy with an experiment determining MICs for tuberculosis. I am using the MABA method and 96 well microtiter plates. The antibiotic I am using is a cationic cyclic decapeptide with a fatty acid tail. It is amphiphatic. So my question is, what type of plate should I be using for the most accurate result. In know some plates are made from PS so their walls in the wells are hydrophobic. So will this have an effect on my MICs i.e. my antibiotic might be attracted to the walls of the well? But then again maybe my cells as well? So do I get a coated plate or what type of plate? Since I am working with TB I have long incubation periods of up to a week :/ thanks for the input


All times are GMT. The time now is 07:12 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09039 seconds with 11 queries