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Real Time PCR machine, which one to choose?

Real Time PCR machine, which one to choose? - Lab Equipment Discussion and Reviews

Real Time PCR machine, which one to choose? - Discuss, ask questions, and post reviews on Lab Equipment. PCR machines, electrophoresis, centrifuges etc.


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  #1  
Old 10-22-2008, 11:18 PM
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Default Real Time PCR machine, which one to choose?



Dear Friends,
I am in the process of purchasing a real time PCR machine. Right now I am debating which one to choose. I have two machines in mind: the CFX96 from BioRad and the RealPlex 4 from Eppendorf. Does any one have any impressions, comments, opinions, reviews that could help me in to decide?
Thank you so much!
All the best
Raul
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  #2  
Old 10-22-2008, 11:33 PM
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Default Re: Real Time PCR machine, which one to choose?

Both BioRad and Eppendorf make good machines, I'd go with BioRad.
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Old 10-25-2008, 10:32 AM
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Default Re: Real Time PCR machine, which one to choose?

Hi there!
Thank you for your post and opinion!
Cheers
Raul Bettencourt
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Old 11-11-2008, 11:38 AM
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Default Re: Real Time PCR machine, which one to choose?

But why the opinion about BioRad equipment wasn't justified?
I personally have never used BioRad real time system, so I don't know much about it but I would use some of others knowledge ;-)
I use LightCycler 2.0 and 480 by Roche and my friend uses Eppendorf system (don't remember the exact model) and both are reliable and easy to work with and have the possibility of automation.
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Old 11-13-2008, 08:11 PM
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Default Re: Real Time PCR machine, which one to choose?

Indeed the BioRad choice was not justified but I would guess it is a personal preference rather than a choice based in techical performances of the machine itself. Trying to use other's knowledge is exactly why I signed up for this forum hoping I would have lots of comments and opinions of users like yourself. Thanks for your comment though.
Raul
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Old 07-24-2009, 02:44 AM
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Default Re: Real Time PCR machine, which one to choose?

The Biorad is more expensive but it gives very good data and tight replicates, and seems to be more sensitive because ct value come up earlier. Both software is easy to use and both having gradient.

YuWei
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Old 10-03-2009, 09:17 AM
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Default Re: Real Time PCR machine, which one to choose?

Hey guys,

i want to set up a molecular biology lab in Greece. Although i havent got a clue from where could i order all the instruments and machines!!any help?
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Old 10-03-2009, 09:18 AM
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Default Re: Real Time PCR machine, which one to choose?

Hey guys,

i want to set up a molecular biology lab in Greece. Although i havent got a clue from where could i order all the instruments and machines!!any help?
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Old 10-30-2009, 01:19 AM
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Default Re: Real Time PCR machine, which one to choose?

you could order them from Bio-Rad.
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Old 12-23-2009, 08:10 PM
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Default Re: Real Time PCR machine, which one to choose?

the same problem of setting up the lab in Russia ))
at 1st you'd better to see the experience from other similar labs, then you choose the vendor and begin discussing pro et contra for each equipment position - yeah, it's really time-consuming task!
Wish you success!
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