Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Products and Vendor Discussion > Molecular Biology Products and Vendors > Lab Equipment Discussion and Reviews
Register Search Today's Posts Mark Forums Read

Lab Equipment Discussion and Reviews Discuss, ask questions, and post reviews on Lab Equipment. PCR machines, electrophoresis, centrifuges etc.


Real Time PCR Machines Thermocyclers

Real Time PCR Machines Thermocyclers - Lab Equipment Discussion and Reviews

Real Time PCR Machines Thermocyclers - Discuss, ask questions, and post reviews on Lab Equipment. PCR machines, electrophoresis, centrifuges etc.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-21-2007, 04:36 AM
Pipette Filler
Points: 1,922, Level: 27 Points: 1,922, Level: 27 Points: 1,922, Level: 27
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2007
Location: EL SALVADOR, CENTRO AMERICA
Posts: 29
Thanks: 0
Thanked 0 Times in 0 Posts
Question Real Time PCR Machines Thermocyclers



Hello Every One

I Am Look For One Real Time Machine

I See Diferent Mark.

My Option Is

1 Smart Cicler .... Cephy

2 Aplaid 7000 ..........apple. Bios.

3 Ligth Cicler ......... Roche

Any Guy Will Recommend One


Thank You Very Much For You Time


Magui
Reply With Quote
  #2  
Old 07-26-2007, 04:58 PM
danfive's Avatar
Nobel Laureate
Points: 8,612, Level: 64 Points: 8,612, Level: 64 Points: 8,612, Level: 64
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2007
Location: Houston TX
Posts: 969
Thanks: 12
Thanked 184 Times in 154 Posts
Default Re: Real Time PCR Machines Thermocyclers

Applied Biosystems plus Taqman probes---great PCR tools, used them in a state certified diagnostic lab.
Reply With Quote
  #3  
Old 07-27-2007, 12:09 AM
Pipette Filler
Points: 1,922, Level: 27 Points: 1,922, Level: 27 Points: 1,922, Level: 27
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2007
Location: EL SALVADOR, CENTRO AMERICA
Posts: 29
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Real Time PCR Machines Thermocyclers

Thank You Danfive

For You Word. We Are Look To Purcharse
Machine For Pcr Real Time.

I Have One Qustion For You
The Taqman Probe Will To Make Disotiation Curve.

Thank You For You Time

Margarita
Reply With Quote
  #4  
Old 07-27-2007, 05:23 PM
danfive's Avatar
Nobel Laureate
Points: 8,612, Level: 64 Points: 8,612, Level: 64 Points: 8,612, Level: 64
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2007
Location: Houston TX
Posts: 969
Thanks: 12
Thanked 184 Times in 154 Posts
Default Re: Real Time PCR Machines Thermocyclers

Taqman probes will not make dissociation curves (melting point curves) as far as I know, that works with SYBR Green (which you can do instead of Taqman probes). Taqman probes are very specific, so they are not really prone to need verification by acquiring melting point of PCR product. In actuality you have primers (forward and reverse) that are specific to your PCR target, and you also have a third complimentary Oligo (the labeled probe). So your Real time reactions and signal are due to both primers and probes binding to the right PCR target sequence.
Reply With Quote
  #5  
Old 07-29-2007, 09:52 PM
Pipette Filler
Points: 1,922, Level: 27 Points: 1,922, Level: 27 Points: 1,922, Level: 27
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2007
Location: EL SALVADOR, CENTRO AMERICA
Posts: 29
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Real Time PCR Machines Thermocyclers

Thank You Dan.

I Read You Explain About Taqman. I See In Diferentes Kit For

Rt Pcr In Real Time Use More Taqman And No Other

Sondas Will Work Whit Curve Of Disocation.

Maybe Is More Shipp ...

Thank You

Margarita
Reply With Quote
Reply

Tags
machines , pcr , real , thermocyclers , time


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
What is Gravity? sdr@sdrodrian.com Physics Forum 1 01-27-2008 02:53 PM
the absolutely final complete collection of ideas blochee Physics Forum 2 06-15-2007 06:31 AM
the absolutely final complete collection of ideas blochee Physics Forum 0 06-14-2007 10:30 PM
Physicists Losing Their Grip?? Consc Physics Forum 218 01-06-2005 01:20 PM


All times are GMT. The time now is 04:11 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.19440 seconds with 16 queries