I am going to establish a conditional gene knockout mouse model. The ES cell is available in KOMP. The allele structure is showed below.
KOMP suggest crossing with Flp mouse to remove lacZ--loxP--neo sequence first , and then crossing with Cre mouse to knockout the gene exon. However, I want to try crossing with Cre mouse to remove the gene exon.
My questions are:
1. Is it possible?
2. What problems should I take a care of?