| |||||||
| Register | Blogs | FAQ | Members List | Calendar | Search | Today's Posts | Mark Forums Read |
| Knockout Mouse Forum Discuss and post questions about knockout mice, transgenic mice and other transgenic animal models. |
tagging proteins in mice
Knockout Mouse Forum
Discuss and post questions about knockout mice, transgenic mice and other transgenic animal models.
 |
 |
| | LinkBack | Thread Tools | Display Modes |
09-28-2007, 02:44 PM
| |||||
| |||||
 tagging proteins in mice I would like to N terminal tag my endogenous protein in mice by targeting it with homologous recombination in ES cells. I am worried that the addition of the tag will change the KOZAK sequence, and affect the translation. This is especially a concern since in normal cells, two alternative translation products can be detected. Does anybody know if tagging a protein effects its translation due to a change in the sequence around the first ATG?        |
| Today
| ||||
| ||||
 Sponsored Links |
|
| Thread Tools | |
| Display Modes | |
Linear Mode
| |
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Nude mice immunity | julyfrombrest | Animal and Molecular Model Systems | 0 | 02-21-2008 08:38 AM |
| SV40 driven transgenic mice question | John O'Neil | Knockout Mouse Forum | 0 | 02-15-2008 08:46 AM |
| SDS-PAGE of Secreted Proteins | liono | SDS-PAGE Gel Electrophoresis Forum | 1 | 01-13-2008 04:06 PM |
| Loss of integrin ?v?8 on dendritic cells causes autoimmunity and colitis in mice | admin | Science News and Views | 0 | 08-30-2007 04:50 PM |
| Sperm proteome gives “tantalising glimpse” towards the origin of sex | kiki06 | Proteomics Forum | 0 | 11-13-2006 09:34 PM |
All times are GMT. The time now is 09:50 AM.
