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Knockout Mouse Forum Discuss and post questions about knockout mice, transgenic mice and other transgenic animal models.


Step tag question

Knockout Mouse Forum

Discuss and post questions about knockout mice, transgenic mice and other transgenic animal models.



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Old 05-22-2007, 10:14 AM
Pipette Filler
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Default Step tag question

Hello,

I was wondering if anyone here has ever expressed a strep tag fusion protein in a transgenic mouse? We have a construct that we would like to overexpress in mouse skin and currently it is in a construct that introduces a strep tag. However I'm not convinced it's ideal for our purposes since I would want to analyse sections by immunohistochemistry and I'm struggling to find antibodies against the strep tag that are suitable for IHC. Does anyone know of a good antibody for this purpose or would we be better off changing the tag?

Many thanks

Sally
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