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Knockout Mouse Forum Discuss and post questions about knockout mice, transgenic mice and other transgenic animal models.


Step tag question

Step tag question - Knockout Mouse Forum

Step tag question - Discuss and post questions about knockout mice, transgenic mice and other transgenic animal models.


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Old 05-22-2007, 10:14 AM
Pipette Filler
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Default Step tag question



Hello,

I was wondering if anyone here has ever expressed a strep tag fusion protein in a transgenic mouse? We have a construct that we would like to overexpress in mouse skin and currently it is in a construct that introduces a strep tag. However I'm not convinced it's ideal for our purposes since I would want to analyse sections by immunohistochemistry and I'm struggling to find antibodies against the strep tag that are suitable for IHC. Does anyone know of a good antibody for this purpose or would we be better off changing the tag?

Many thanks

Sally
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