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I'm new here, but I was just wondering if anyone could help me with a few questions.
With the use of homologous recombination to knockout endogenous genes in the mouse are there possibilities of having more than one copy of the target gene entering the genome? And how efficient is this method in trying to knockout genes in the mouse?
Thanks in advance.
Re: Homologous recombination
I did once and the effiency is quite low, it is around 0.5-1% for the real HR event. In that time, I pick up 400 clones, and got two HR positive clones. The efficiency is also dependent on the locus.
|homologous , recombination|
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