Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Knockout Mouse Forum
Register Search Today's Posts Mark Forums Read

Knockout Mouse Forum Discuss and post questions about knockout mice, transgenic mice and other transgenic animal models.


Homologous recombination

Homologous recombination - Knockout Mouse Forum

Homologous recombination - Discuss and post questions about knockout mice, transgenic mice and other transgenic animal models.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-21-2007, 12:11 AM
Pipette Filler
Points: 1,343, Level: 21 Points: 1,343, Level: 21 Points: 1,343, Level: 21
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2007
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default Homologous recombination



Hi,

I'm new here, but I was just wondering if anyone could help me with a few questions.

With the use of homologous recombination to knockout endogenous genes in the mouse are there possibilities of having more than one copy of the target gene entering the genome? And how efficient is this method in trying to knockout genes in the mouse?

Thanks in advance.
Reply With Quote
  #2  
Old 06-12-2007, 03:14 PM
Pipette Filler
Points: 1,584, Level: 23 Points: 1,584, Level: 23 Points: 1,584, Level: 23
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2006
Posts: 7
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Homologous recombination

I did once and the effiency is quite low, it is around 0.5-1% for the real HR event. In that time, I pick up 400 clones, and got two HR positive clones. The efficiency is also dependent on the locus.
Reply With Quote
Reply

Tags
homologous , recombination


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Red/ET recombination Tam Protocols and Methods Forum 2 02-26-2009 02:13 PM
Low Homologous Recombination Rates Stem Cells wexfrdo Stem Cell Forum 1 07-23-2008 12:19 PM
Question on recombination in E.coli and in baculovirus DK Protocols and Methods Forum 3 03-03-2008 04:34 PM
Illegitimate Recombination aftabac Omics and Genomics Forum 2 12-02-2006 06:10 PM
Recombination Models genzeryk Cell Biology and Cell Culture 1 05-22-2006 02:09 AM


All times are GMT. The time now is 10:27 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11402 seconds with 16 queries