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IP buffer: K-HEPES or HEPES-KOH?

IP buffer: K-HEPES or HEPES-KOH? - Immunoprecipitation Forum

IP buffer: K-HEPES or HEPES-KOH? - IP or Immunoprecipitation Forum.


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Old 04-11-2014, 06:33 PM
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Default IP buffer: K-HEPES or HEPES-KOH?



Hi everyone!
I'm preparing a buffer for IP: 20mM K-HEPES pH 7.4, 110mM KOAc, 2 mM MgCl2, 1% Triton, 250 Mm NaCl. The question is whether K-HEPES, which is available as powder, can be substituted by normal HEPES and adjusting pH by KOH? Are K-HEPES and HEPES-KOH the same substance?
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Old 04-12-2014, 03:19 PM
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Default Re: IP buffer: K-HEPES or HEPES-KOH?

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent; one of the twenty Good's buffers with pKa (25 C) = 7.5 and with useful pH range from 6.8 to 8.2. Exist two salt form: sodium and potassium Fluka cod. 54466 and 54464 respectively. The K-HEPES from you indicated is probably the HEPES potassium salt
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Old 04-13-2014, 02:37 PM
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Default Re: IP buffer: K-HEPES or HEPES-KOH?

Hepes-K 20 mM at pH 7.4 : dissolve 5.528 g HEPES potassium salt (m.w. 276.4) in 800 ml dH2O. Adjust pH to 7.4 with 20 mM KOH, then dissolve others ingredients, and to make a final volume of 1 liter.
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