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Non-reducing IP and agilent

Non-reducing IP and agilent - Immunoprecipitation Forum

Non-reducing IP and agilent - IP or Immunoprecipitation Forum.

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Old 06-12-2012, 07:19 AM
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Default Non-reducing IP and agilent

I have to analyze proteins on Agilent 2100 Bioanalyzer. They should be purified by immunoprecipitation. The problem is that those two proteins are of the same mass as light and heavy chain of antibody, so I have to do experiment in non-reducing conditions. In that way, I hope I will see both my proteins and L-H chains together. Is there a way to do that? How to choose elution buffer, which is compatible with Agilent chip?
And will the antibodies denaturize by boiling?
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