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Heavy/light chain problem in IP/WB

Heavy/light chain problem in IP/WB - Immunoprecipitation Forum

Heavy/light chain problem in IP/WB - IP or Immunoprecipitation Forum.


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Old 03-20-2012, 06:17 AM
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Default Heavy/light chain problem in IP/WB



Hi all,
I have a common question regarding the heavy chain and light chain detected in IP/WB that I haven't found a good answer yet.
I don't want to crosslink my IP antibody to beads because it may decrease the binding efficiency. Therefore I'm considering the following options instead of 2nd antibody:
A. pierce clean blot detecting native IgG
B. Trueblot from ebioscience
C. HRP conjugated Protein A or G from GE Health.

I was wondering if anyone has compared these products and what's your opinion.
And does anyone know if clean-blot and true-blot are also HRP conjugated protein A or G? Pierce and ebioscience didn't mention how their products work in their data sheet

Last edited by jongleur; 03-20-2012 at 06:18 AM.
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