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A question about Serial IP

A question about Serial IP - Immunoprecipitation Forum

A question about Serial IP - IP or Immunoprecipitation Forum.

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Old 08-17-2011, 03:17 AM
Dic Dic is offline
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Default A question about Serial IP

I am trying to identify the protein complex by immunoprecipitation using two different antibodies. What is the best way to elute the complex after first IP? Can I use 100 mM Glycine (pH2.5)? Or I have to elute with the corresponding antigen? Anybody have an idea or protocol for my ref? Thanks!
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