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#1
03-28-2011, 02:32 PM
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 Removing heavy/light chains in IP blots I'm trying to rid heavy chains from my IP. I have a mouse monoclonal antibody to ip my protein of interest, and I want to see the interaction with a protein of 22KDa (runs at light chain level) and one protein of 60KDa (at heavy chain level more or less). What do you recommend me to do?I have read a lot of things...I'm a little bit confused....  Thanks in advance!!!  |
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#3
08-12-2011, 11:55 PM
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| Re: Removing heavy/light chains in IP blots To avoid detection of the heavy chain use a light chain specific secondary Ab. Likewise use a Fc fragment specific secondary Ab to avoid detection of the light chain. |
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| blots , chains , heavy chain , heavy or light , immunoprecipitation , removing |
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