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Removing heavy/light chains in IP blots

Removing heavy/light chains in IP blots - Immunoprecipitation Forum

Removing heavy/light chains in IP blots - IP or Immunoprecipitation Forum.


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Old 03-28-2011, 02:32 PM
Pipette Filler
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Default Removing heavy/light chains in IP blots



I'm trying to rid heavy chains from my IP. I have a mouse monoclonal antibody to ip my protein of interest, and I want to see the interaction with a protein of 22KDa (runs at light chain level) and one protein of 60KDa (at heavy chain level more or less). What do you recommend me to do?I have read a lot of things...I'm a little bit confused....
Thanks in advance!!!
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Old 05-19-2011, 04:24 AM
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Default Re: Removing heavy/light chains in IP blots

use a primary ab that is non-mouse for detection.
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Old 08-12-2011, 11:55 PM
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Default Re: Removing heavy/light chains in IP blots

To avoid detection of the heavy chain use a light chain specific secondary Ab. Likewise use a Fc fragment specific secondary Ab to avoid detection of the light chain.
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blots , chains , heavy chain , heavy or light , immunoprecipitation , removing


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