| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Immunoprecipitation Forum IP or Immunoprecipitation Forum. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
03-02-2011, 08:38 PM
| |||||||||||
| |||||||||||
 How would immunoprecipitated proteins look like in an SDS-page experiment? I have 12 different proteins that have been immunoprecipitated and I was wondering how they would look like. Thanks.  |
|
#2
03-03-2011, 07:14 PM
| ||||||||||||
| ||||||||||||
| Re: How would immunoprecipitated proteins look like in an SDS-page experiment? The immunoprecipitated (IP) proteins' migration in the gel should be delayed in comparison to non-IP proteins. This because the bound antibody causes an increase of the molecular weight of the IP protein thus delaying its migration. However, SDS-page electrophoresis needs denaturation (boiling) of the proteins prior running. This will also denature the bound antibody and you will see two bands (one of your target protein and one of the antibody) instead of one; in which case the delaying won't be seen. |
| The Following User Says Thank You to luisillo For This Useful Post: | ||
admin (03-04-2011)
| ||
|
| Tags |
| experiment , immunoprecipitated , proteins , sdspage |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Proposition for performing a series of electromagnetic experiments | Hamid.V.Ansari@gmail.com | Physics Forum | 22 | 09-16-2007 02:52 AM |
| Proposition for performing a series of electromagnetic experiments | Hamid.V.Ansari@gmail.com | Physics Forum | 0 | 09-05-2007 05:41 AM |
| Moving Dimensions Theory--Returning Physical Models to Physics & Unifying QM & SR & GR & Statistical Mechanics | Captain Ranger McCoy | Physics Forum | 8 | 08-29-2007 07:00 PM |
| the absolutely final complete collection of ideas | blochee | Physics Forum | 2 | 06-15-2007 06:31 AM |
| the absolutely final complete collection of ideas | blochee | Physics Forum | 0 | 06-14-2007 10:30 PM |
Linear Mode
