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Strep-tactin magnetic beads

Strep-tactin magnetic beads - Immunoprecipitation Forum

Strep-tactin magnetic beads - IP or Immunoprecipitation Forum.


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  #1  
Old 11-08-2010, 09:01 PM
Pipette Filler
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Default Strep-tactin magnetic beads



Hi,
I try to detect binding partners of my strep-tagged protein. The binding of my construct with strep magnetic beads works fine but I fail to detect other partners proteins. I use Nacl-tris-NP40 for washing, I wonder if it could remove my partners from my construct by modifying the binding. Which washing buffer do you use in your CoIP usually? under native conditions of course.
Thanks in advance
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Old 05-12-2011, 08:03 PM
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Default Re: Strep-tactin magnetic beads

Explore our extensive collection of beads, findings, gemstones, charms, and jewelry supplies for discriminating bead artists
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Old 05-13-2011, 04:18 PM
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Thumbs down Re: Strep-tactin magnetic beads

very usefull
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Old 08-10-2012, 08:17 AM
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Default Re: Strep-tactin magnetic beads

Hi,
I actually wanted to ask you which magnetic beads are you using and if they´re working. I also want to purify my protein with STREP-tactin but I am using columns and the process is very time consuming. I would like to try the magnetic beads.
Concerning the binding partners, have you tried to change the NaCl and NP-40 concentrations?
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