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Preclear Step

Preclear Step - Immunoprecipitation Forum

Preclear Step - IP or Immunoprecipitation Forum.

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Old 06-10-2010, 06:24 PM
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Default Preclear Step

Hi everyone,

I'm fairly new to ChIP and am working through my first run at the protocol. Basically, when I was running through the preclear step, I was incubating for 30 minutes, ran out for a coffee, and when I got back, I added my antibody to my samples without first spinning down and removing the supernatant. So I ended up incubating all night with my agarose beads, and antibody in my DNA sample. I was wondering how crucial it is to remove the beads?

woops, wrong forum. Sorry everyone.

Last edited by BakedGoat; 06-10-2010 at 06:31 PM. Reason: wrong forum
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