Originally Posted by deeps
I am doing the immunoprecipitation of secretory proteins from culture medium and looking for a loading control for these secretory ptns.My P.I was telling that erk is a membrane ptn and I cann't use it for my purpose.So does anybody have any idea about it?
me too, I would like to do an IP on conditioned medium. I'm planning to incubate the medium with my cells for a couple of days, concentrate the medium with Amicons and then do IP...
My Question is... Do you add FCS to the medium in which you collect your secreted protein??? Does it not interfere with IP?
I would be grateful about an answer, thank you!