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deeps 06-02-2010 05:27 PM

Measuring protein concentration after adding SDS sample buffer
 
Hi all,

I am doing the immunoprecipitation of secreted proteins from the culture medium and I was also able to capture my protein of interest but I am unable to quantify the protein amount as I mix the agarose beads with the 2xSDS sample buffer and boil it.Can any one lemme know how I can measure the protein concentration after adding sample buffer?

Thank You

danfive 06-02-2010 08:23 PM

Re: Measuring protein concentration after adding SDS sample buffer
 
Check out Reducing Agent Compatible Protein Quantitation Kits

Pierce is excellent, with compatibilities up to 5% SDS (regular kit) and 10% SDS (Reducing Agent compatible kit.

-link to the comparison chart---> [Only registered and activated users can see links. Click Here To Register...]

Link to product ---> [Only registered and activated users can see links. Click Here To Register...]

Good luck.

Vaishali 02-24-2011 06:32 AM

Re: Measuring protein concentration after adding SDS sample buffer
 
Would the cheapest way be to run out the same amounts of all your samples and do a western and use a densitometer? I'm new to this stuff but thought I put that out there.


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