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Immunoprecipitation of small, transmembrane proteins

Immunoprecipitation of small, transmembrane proteins - Immunoprecipitation Forum

Immunoprecipitation of small, transmembrane proteins - IP or Immunoprecipitation Forum.


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Old 11-25-2009, 06:31 PM
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Default Immunoprecipitation of small, transmembrane proteins



I am trying to IPP from cell lysates two small (9-12kDa) transmembrane proteins, with no success to date. I suspect my problem stems from the transmembrane region leading to poor solubility and possibly protein aggregation.

Does anyone have a protocol, or advice, on how to optimize such a system?

Thanx

Bryan
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