|Register||Search||Today's Posts||Mark Forums Read|
|Immunoprecipitation Forum IP or Immunoprecipitation Forum.|
| ||LinkBack||Thread Tools||Display Modes|
nonspecific bands in silver stain IPs
I'm trying to precipitate my protein of interest from total cell lysate, along with interacting proteins, so that they are visible on silver stained gels. The problem is that I am getting a lot of nonspecific bands sticking to control IPs (beads alone or with nonrelated antibody).
I have tried preclearing, and using higher ultracentrifugation, which have improved the stain a bit, but still too many nonspecific bands. Any suggestions? I'm about to try washing with different salt concentrations (usually use lysis buffer with 1% triton and 150mM NaCl).
|bands , ips , nonspecific , silver , stain|
|Thread||Thread Starter||Forum||Replies||Last Post|
|How to avoid Nonspecific bands on multiplex PCR?||Maddy||PCR - Polymerase Chain Reaction Forum||3||02-24-2010 08:28 AM|
|Immidiate dissappearing of bands on DNA sequencing gels after silver staining||himanshu||SDS-PAGE Gel Electrophoresis Forum||2||07-24-2008 06:17 PM|
|ladder for silver stain||Marzia Barbieri||Protocols and Methods Forum||0||08-31-2005 10:15 AM|
|Various questions about Silver stain||Fabius||Protocols and Methods Forum||0||03-07-2004 01:06 PM|
|anti-HA Western / Immunoblot nonspecific bands||Andrew||Protocols and Methods Forum||3||01-22-2004 06:07 AM|