I want to deplete 15 mL of human and sheep plasma for ceruloplasmin.
I have been looking up methods but I'm confused about how do go about it.
Most people seem to follow this protocol: incubate plasma with an antibody raised against ceruloplasmin. Add IgA and/or IgG conjugated agarose beads. Incubate and pellet the beads.
But for 15 mL of plasma I would need a lot of expensive antibody.
Is there a protocol and/or product anyone can recommend? A bead system, or maybe an affinity column I can load with a specific antibody, another method? Or recommend where I should be looking for these answers?
Thank you for any suggestions.