| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Immunoprecipitation Forum IP or Immunoprecipitation Forum. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
02-22-2009, 05:26 AM
| |||||||||||
| |||||||||||
 Pulldown of structural proteins Hello. I would appreciate if anyone can help me. From the yeast 2 hybrid results, the protein of my interest seems to interact with structural proteins in muscle and heart. Because there is no cell line currently avalilable that express structural proteins abundantly, I am planning to clone my gene into a vector with histidine tag and use Pierce’s product “Pull-Down PolyHis Protein:Protein Interaction Kit (Catalog Number 21277)”. Here is what I am planning: Bait: clone in the gene of my interest into pcDNA(tm) 3.1/V5-His (Invitrogen);, which attaches 6x His and V5 at the C-terminus, and overexpress it in HEK293T cells. Prey: skeletal muscle from the thigh of a mouse. (tissue extract) Does anyone try such an approach? Or any other approach? Thank you very much for your help in advance. Shizuka  |
|
| Tags |
| proteins , pulldown , structural |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Human Cytome Project - Update 24 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 1 | 08-01-2010 02:18 PM |
| Human Cytome Project - an idea - Update 19 April 2005 | Peter Van Osta | Cell Biology and Cell Culture | 1 | 06-01-2009 02:17 PM |
| First African Structural Biology Conference (FASB 2006) | FASB 2006 Organisers | Protein Forum | 0 | 07-21-2006 08:37 PM |
| A Human Cytome Project - an idea - Update 14 March 2005 | Peter Van Osta | Cell Biology and Cell Culture | 0 | 03-14-2005 01:27 PM |
| Human Cytome Project - Update 6 Jan. 2005 | Peter Van Osta | Cell Biology and Cell Culture | 0 | 01-06-2005 10:18 AM |
Linear Mode
