Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Immunoprecipitation Forum (http://www.molecularstation.com/forum/immunoprecipitation-forum/)
-   -   Co-Immunoprecipitation Co-IP Problems (http://www.molecularstation.com/forum/immunoprecipitation-forum/56089-co-immunoprecipitation-co-ip-problems.html)

tonyz06 01-28-2009 04:16 AM

Co-Immunoprecipitation Co-IP Problems
 
Hey dudes,

when i do co-ip experiments, I use normal mouse igG as the negative control, but surprisingly, my protein of interest was immunoprecipitated with normal mouse igG!

any ideas why?

thanks

admin 01-28-2009 04:17 AM

Re: Co-Immunoprecipitation Co-IP Problems
 
hey tony,
have you thought of non specific interactions that may competing with your protein of interest?
do you have detergent in your incubation and wash buffer? maybe you could also increase the concentration in salts?


All times are GMT. The time now is 05:30 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08915 seconds with 11 queries