Co-Immunoprecipitation Co-IP Problems
when i do co-ip experiments, I use normal mouse igG as the negative control, but surprisingly, my protein of interest was immunoprecipitated with normal mouse igG!
any ideas why?
Re: Co-Immunoprecipitation Co-IP Problems
have you thought of non specific interactions that may competing with your protein of interest?
do you have detergent in your incubation and wash buffer? maybe you could also increase the concentration in salts?
|All times are GMT. The time now is 10:22 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved