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#1
01-13-2009, 11:45 AM
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 Immunoprecipitation Troubleshooting Hi everyone, I am currently using/working on a protein that is not readily solubilized in RIPA. Therfore I need to add urea to RIPA in order to solubilize this protein. My question is whether or not I can use urea lysate for IP. Also how much should I dilute the urea so that it is compatible with immunoprecipitation by protein A/G beads? Thanks  |
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