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pipette_man 01-13-2009 12:45 PM

Immunoprecipitation Troubleshooting
 
Hi everyone,
I am currently using/working on a protein that is not readily solubilized in RIPA. Therfore I need to add urea to RIPA in order to solubilize this protein.
My question is whether or not I can use urea lysate for IP. Also how much should I dilute the urea so that it is compatible with immunoprecipitation by protein A/G beads?

Thanks


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