| | Re: problems about western blot after Co-IP
Thank you for help. I just don't know it is a new product. If it really works so well, it will help me a lot. I don't know how to insert the figure here. I can describe my problem first.
The secondary antibody of the antibody I used to do my co-ip is anti-rabbit. I want to
check some interaction proteins by WB after co-ip, so I just test the inference of secondary antibody in my lab first. I have loaded the same IP sample to three lanes and just add different secondary antibody to see the results. Of course, the contamination of anti-rabbit secondary antibody is very serious, besides the position of heavy chain and light chain, many other bands existed. For the anti-mouse and anti-goat secondary antibody, there are also bands existed on the position of heavy chain. So this problem will bring a lot of trouble for my WB experiments after co-ip. May be the Clean-Blot IP Detection Reagent can help me to solve this problem. Or may be some one can give me some other suggestions.