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Beta-Actin Loading Control Immunoprecipitation

Beta-Actin Loading Control Immunoprecipitation - Immunoprecipitation Forum

Beta-Actin Loading Control Immunoprecipitation - IP or Immunoprecipitation Forum.

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Old 09-16-2008, 02:46 AM
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Question Beta-Actin Loading Control Immunoprecipitation

Hi can we use beta-actin as loading control if we do an Immunoprecipitation? if no why?
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Old 07-16-2009, 11:22 PM
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Default Re: Beta-Actin Loading Control Immunoprecipitation

i would use B-actin for the input gel (10% of lysate for ex) To see the IP efficiency I would rather blot with the antibody for the protein that I did the IP for.
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beta-actin , betaactin , control , immunoprecipitation , loading , loading control

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