Immunoprecipitation after Co-Transfection
Hello all. I am doing an experiment and need some help. I will IP 2 proteins with different tags expressed in HEK 293T cells.
I am going to co-transfect the 2 vectors into the cells however these two proteins are expressing at different efficiency!
One is not expressing too wel. How can I transfect the two and examine them if one is very low. Maybe one is decreasing the efficiency of the other?
does anyone have similar problems or any solutions?
|All times are GMT. The time now is 11:51 PM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved