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26x900mm SE columns

26x900mm SE columns - Immunoprecipitation Forum

26x900mm SE columns - IP or Immunoprecipitation Forum.

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Old 07-09-2008, 07:44 AM
Pipette Filler
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Default 26x900mm SE columns

Hello everybody,

I have a doubt about a protocol for IgM purification.

The protocol suggest a column of 26x900mm, but I have in my lab columns 0,8x4cm with a pore 30um. It's necessary that specific columns for purification with Sepharose 6B or I could use the columns I have at my disposal in may lab.

An other question is, we can choose two kind of sepharose 6B for purification a powder preparations or beads, which one is better for purification?

Thank you very much, I apologise my english I don't know if I have explain my trouble

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