Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Antibody Forum > Immunoprecipitation Forum
Register Search Today's Posts Mark Forums Read

Immunoprecipitation Forum IP or Immunoprecipitation Forum.


hsp90 immunoprecipitation

hsp90 immunoprecipitation - Immunoprecipitation Forum

hsp90 immunoprecipitation - IP or Immunoprecipitation Forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 05-16-2008, 02:30 PM
Pipette Filler
Points: 508, Level: 10 Points: 508, Level: 10 Points: 508, Level: 10
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2008
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default hsp90 immunoprecipitation



Hy everybody,
I try to immunoprecipitate Hsp90 from tumoral cell lysate; i try different antibidies and different protocoles but il stil doesent work.
Does anybody immunoprecipitate Hsp90 from tumoral cell?
Thanks for answering

Carole
Reply With Quote
  #2  
Old 05-16-2008, 02:40 PM
danfive's Avatar
Nobel Laureate
Points: 8,612, Level: 64 Points: 8,612, Level: 64 Points: 8,612, Level: 64
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2007
Location: Houston TX
Posts: 969
Thanks: 12
Thanked 184 Times in 154 Posts
Default Re: hsp90 immunoprecipitation

Done IP successfully with her2 (about 1% of mass of BC tumor cells). Is hsp90 overexpressed in your cell line?
My guess is that if your Ab works well in westerns it should work in IP as long as you have enough target protein in the first place. Have you tried it out on Western Blots? Antibodies etc are new or freshly made? Incubation times are reasonable?
Reply With Quote
Reply

Tags
hsp90 , immunoprecipitation


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Heavy Light Chain Immunoprecipitation Problems IoDiNE Immunoprecipitation Forum 3 05-19-2011 04:08 AM
Immunoprecipitation atahualpa Protocols and Methods Forum 3 02-16-2007 03:50 PM
Immunoprecipitation atahualpa Protein Forum 3 02-16-2007 03:50 PM
Immunoprecipitation atahualpa Cell Biology and Cell Culture 0 05-19-2006 04:26 PM
Immunoprecipitation without beads? Ben Long Protocols and Methods Forum 6 03-13-2005 09:51 PM


All times are GMT. The time now is 01:58 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11433 seconds with 16 queries