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Avoid degradation in PMN isolation

Avoid degradation in PMN isolation - Immunology and Host-Pathogen Interactions

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Old 10-16-2012, 02:37 PM
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Default Avoid degradation in PMN isolation



Hello!

Im starting a proyect which consists in histone analysis of neuthropil. But I've seen some degradation in H3 and H4 due to the method of neutrophil isolation. How I could avoid this degradation?

I use protease inhibitors during all the process but is not enought

Thank you.
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