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Deparaffinization, tissue incubation over night in 85 % ethanol

Deparaffinization, tissue incubation over night in 85 % ethanol - Immunohistochemistry Forum

Deparaffinization, tissue incubation over night in 85 % ethanol - Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections.


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Old 06-28-2013, 07:59 PM
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Default Deparaffinization, tissue incubation over night in 85 % ethanol



Hi all!

I have a question about the deparaffinization procedure of FFPE tissue. Our lab assistant did the deparaffinization as followed:
- Paraffin melting in the oven (55 ˚C)
- Addition of Xylene, removal of Xylene/ paraffin
- Incubation in Xylene for 30 min
- Incubation in 100 % Ethanol for 30 min
- Incubation in 100 % Ethanol for 30 min
- Incubation in 95 % Ethanol for 30 min

Then, he added 85 % Ethanol and forgot the samples so that they were sitting in 85 % Ethanol overnight at room temperature.
So, what do you think, is the tissue still usable or should I start over again? My aim is not to do histological staining but to extract proteins from the tissue.

Thanks for your help!

Steffi
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deparafinization , ffpe , formalin-fixation


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