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Primary Antibody incubation time...too long?

Primary Antibody incubation time...too long? - Immunohistochemistry Forum

Primary Antibody incubation time...too long? - Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections.


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Old 09-11-2012, 04:04 AM
Pipette Filler
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Default Primary Antibody incubation time...too long?



Im an intern, and I think I might have made a mistake in the IHC protocol by not washing properly after applying my primary antibody. I was rushing to go home, and I just threw out the solution with the primary and put in PBST solution and left it over a weekend without properly washing. Will I get high background from residual antibody?
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