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Immunohistochemistry Forum Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections.


Pap-Pen problems

Pap-Pen problems - Immunohistochemistry Forum

Pap-Pen problems - Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections.


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  #1  
Old 02-23-2011, 09:58 AM
Pipette Filler
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Default Pap-Pen problems



Hi all,
I used to do a lot of in situ IHC on mouse embryos and had many problems with the pap pen (mainly my Triton x100 used to eat it all away in 5min and my antibodies used to spill...). I tried tween20, still same effect.

I found a new product on the market from a local startup company (invignome) and it worked well for me. The best thing about it is that the pens come in six colors so I used to mark my negative controls with the red marker and that narrowed down the number of "primary antibody, primary antibody... primer... OOPS! - that was(!) the negative control" sort of mistakes.
Just incase you guys suffer from the same issues.
Cheers,
Saar
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  #2  
Old 03-14-2011, 04:02 PM
Pipette Filler
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Default Re: Pap-Pen problems

Thanks for the point!!!
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  #3  
Old 03-15-2011, 09:28 PM
Pipette Filler
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Default Re: Pap-Pen problems

Hi Laura,
Glad I could help.
What do you do with your IHC?
Is there anything else I can help you with?
Regards,
Saar
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