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| Immunohistochemistry Forum Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections. |
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| We are currently in the process of designing a study for looking at the epithelium of the inner and outer foreskin. Several recent studies have looked at keratinization of the inner and out foreskin and found no significant difference. This was using fillaggrin staining and H&E stains. Now correct me if I am wrong, but I would assume that the stratum corneum would be thicker on the outer foreskin compared to the inner. Is there anyway to pick this dead cell layer up over the live keratin layer (I am assuming fillaggrin would stain the live keratin layer?). Any thoughts on the best way to pick up this dead cell layer routinely? |
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| corneum , stain , stratum |
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