Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Histology Forum > Immunohistochemistry Forum
Register Search Today's Posts Mark Forums Read

Immunohistochemistry Forum Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections.


DAB staining problem

DAB staining problem - Immunohistochemistry Forum

DAB staining problem - Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-29-2010, 01:42 PM
Pipette Filler
Points: 854, Level: 16 Points: 854, Level: 16 Points: 854, Level: 16
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2010
Posts: 9
Thanks: 4
Thanked 0 Times in 0 Posts
Default DAB staining problem



Is anyone using 3,3'-Diaminobenzidine (DAB) staining? I am wondering how long do you wait until the samples turn brown? I am using it on paraffin sections. I got good result before (I waited about 15 mins) but now I am having problem with it. I had to wait until 1-2 hours until the samples turn brown... and work on some sections but not all...
So I am wondering if there is something wrong with the DAB solution or perhaps something else... Thank you so much !!
Reply With Quote
  #2  
Old 10-05-2010, 07:34 PM
Pipette Filler
Points: 854, Level: 16 Points: 854, Level: 16 Points: 854, Level: 16
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2010
Posts: 9
Thanks: 4
Thanked 0 Times in 0 Posts
Default Re: DAB staining problem

Uh thank you guys, actually the problem was solved. I tested my method using two different samples (from different studies...) and seems that the new sections from one of the studies were processed wrong... The staining was perfect in the old sections after 15 mins DAB...
Reply With Quote
Reply

Tags
dab , ihc , problem , staining


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Staining Of Tapeworms Razielox Basic Lab Protocols and Techniques 2 03-18-2011 03:02 PM
Another contimation problem helpme Cell Biology and Cell Culture 3 05-18-2010 11:13 PM
SDS-PAGE problem Steve Nothwehr Protocols and Methods Forum 2 10-16-2009 08:59 AM
Unidentified problem with low concentrated samples ggerard Western Blot Forum 3 12-22-2008 05:52 PM
Gus staining Kulbir Singh Arabidopsis and Plant Biology 0 06-20-2008 07:06 PM


All times are GMT. The time now is 01:58 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11598 seconds with 16 queries