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| Immunohistochemistry Forum Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections. |
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#1
02-05-2009, 09:29 PM
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 IHC for formalin fixed cells I am trying to stain cells in matrigel 3 dimensional cultures for E_Cadherin and prostate specific membrane antigen. I fix my cultures ( Cells and matrigel) in formalin then embed them in OCT(Optimum cutting temperatur) making frozen sections from them. If I stain do I need to retrieve the antigen by heating in Citrate solution or not. Thanks, Last edited by admin; 02-06-2009 at 05:27 AM. Reason: no emails please  |
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| cells , fixed , formalin , ihc |
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