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Immunohistochemistry Forum Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections.


IHC for formalin fixed cells

IHC for formalin fixed cells - Immunohistochemistry Forum

IHC for formalin fixed cells - Immunohistochemistry Forum. Post and discuss questions on immunohistochemistry methods including paraffin, frozen, or free floating tissue sections.


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Old 02-05-2009, 10:29 PM
Pipette Filler
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Default IHC for formalin fixed cells



I am trying to stain cells in matrigel 3 dimensional cultures for E_Cadherin and prostate specific membrane antigen. I fix my cultures ( Cells and matrigel) in formalin then embed them in OCT(Optimum cutting temperatur) making frozen sections from them. If I stain do I need to retrieve the antigen by heating in Citrate solution or not.

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Old 04-23-2009, 10:18 PM
Pipette Filler
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Default Re: IHC for formalin fixed cells

After fixing in formalin, you will might need antigen retrieval, but not necessarily.
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